5 years ago

Chemical Protein Synthesis with the α-Ketoacid–Hydroxylamine Ligation

Chemical Protein Synthesis with the α-Ketoacid–Hydroxylamine Ligation
Jeffrey W. Bode
The coupling of an α-ketoacid and a hydroxylamine (KAHA ligation) affords amide bonds under aqueous, acidic conditions without the need for protecting groups or coupling agents. Translating this finding into a general approach to chemical protein synthesis required the identification of methods to incorporate the key functional groups into unprotected peptide segments—ideally using well-established Fmoc solid-phase peptide synthesis protocols. A decade of effort has now led to robust, convenient methods for preparing peptides bearing free or masked C-terminal α-ketoacids and N-terminal hydroxylamines. The facile synthesis of the segments and the aqueous, acidic conditions of the KAHA ligation make it ideal for the construction of small proteins (up to 200 residues), including SUMO and related modifier proteins, betatrophin and other protein hormones, nitrophorin 4, S100A4, and the cyclic protein AS-48.

Publisher URL: http://dx.doi.org/10.1021/acs.accounts.7b00277

DOI: 10.1021/acs.accounts.7b00277

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