Stem Cells and Development
Stem Cells and Development
5 years ago

Angiogenic activity of human adipose-derived mesenchymal stem cells under simulated microgravity.

Ludmila Buravkova, Andrey Ratushnyy, Maria Ezdakova, Danila Yakubets
Multipotent mesenchymal stem/stromal cells (MSCs) are strongly involved in tissue homeostasis mainly through paracrine regulation. Here we examined the influence of simulated microgravity on the angiogenic potential of adipose-derived MSCs (ASCs). The conditioned medium (CM) from RPM-exposed ASCs stimulated the formation of vessel network in ovo, endothelial cell (EC) capillary-like network ex ovo and non-directed EC migration in vitro. These effects were driven by alteration of both angiogenesis-related gene and protein expression. The elevation of angiogenic regulators Serpin E1, Serpin F1, IGFBP, VEGF, IL-8 was detected in ASC-CM after 3D-clinorotation. Besides, transcription of genes encoded growth factors with proangiogenic activity were upregulated including VEGF-с and VEGF-a. These data evidenced that besides direct effect on ECs, microgravity could provoke MSC-mediating specific microenvironment for ECs supporting their functions i.e. proliferation and migration via increased production of IL-8 and VEGF as well as other paracrine factors involved in angiogenesis regulation.

Publisher URL: http://doi.org/10.1089/scd.2017.0262

DOI: 10.1089/scd.2017.0262

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