Journal of Experimental Botany
Journal of Experimental Botany
5 years ago

The 14-3-3 Protein HbGF14a Interacts with a RING Zinc Finger Protein to Regulate the Expression of Rubber Transferase Gene from Hevea Brasiliensis.

Jia-Hong Zhu, Ying Wang, Dong Guo, Hui-Liang Li, Shi-Qing Peng, Zi-Ping Yang
Hevea brasiliensis is a key commercial natural rubber (cis 1,4-polyisoprene) source. In H. brasiliensis, rubber transferase is responsible for cis-1,4-polymerization of isoprene units from isopentenyl diphosphate and implicated in natual rubber yield. Little is known about the regulatory mechanism of rubber transferase gene at molecular levels. Here, we showed that 5'UTR intron of the promoter of rubber transferase gene (HRT2) suppresses the expression of HRT2. A Hevea RING zinc finger protein (designated as HbRZFP1) was able to interact specifically with the HRT2 promoter to down-regulate its transcription in vivo. Hevea brasiliensis 14-3-3 protein (named as HbGF14a) was identified to interact with HbRZFP1, both in yeast and in planta. Transient co-expression of HbGF14a and HbRZFP1-encoding cDNAs led to relieve HbRZFP1-mediated HRT2 transcription inhibition. HbGF14a repressed the protein-DNA binding of HbRZFP1 with the HRT2 promoter in yeast. We conclude a regulatory mechanism by which the binding of 14-3-3 to HbRZFP1 interferes with the interaction of HbRZFP1 with the HRT2 promoter, thereby repressing the protein-DNA binding of HbRZFP1 with the HRT2 promoter. Our studies provide insights into a role of 14-3-3 in mediating expression of rubber transferase gene from Hevea brasiliensis.

Publisher URL: http://doi.org/10.1093/jxb/ery049

DOI: 10.1093/jxb/ery049

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.