Journal of the Science of Food and Agriculture
Journal of the Science of Food and Agriculture
5 years ago

An ultrasensitive chemiluminescence immunoassay for fumonisin B1 detection in cereals based on gold-coated magnetic nanoparticles.

Lie Liu, Peter de B Harrington, Hongquan Zhang, Leiliang He, Fei Yu, Songcheng Yu, Mingsha Jie, Lingbo Qu, Yongjun Wu, Yanqiang Li
In this work, a novel highly sensitive magnetic enzyme chemiluminescence immunoassay (MECLIA) was developed to detect fumonisin B1 (FB1 ) in cereal samples. The gold-coated magnetic nanoparticles (Fe3 O4 @Au, GoldMag) were used as solid phase carrier to develop a competitive CLIA for detecting FB1 , in which FB1 in samples would compete with FB1 -OVA coated on the surface of Fe3 O4 @Au nanoparticles in binding with FB1 antibodies. Successively, horseradish peroxidase labeled goat anti-rabbit IgG (HRP-IgG) was conjugated with FB1 antibodies on the microplate. In substrate solution containing luminol and H2 O2 , HRP-IgG catalyzed luminol oxidation by H2 O2 , generating a high chemiluminescence signal. The FB1 immune GoldMag particles were characterized by Fourier transform infrared (IR) spectroscopy, scanning electron microscope, zeta potential analysis, etc.

Publisher URL: http://doi.org/10.1002/jsfa.8849

DOI: 10.1002/jsfa.8849

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