Comparative study of strong cation exchangers: Structure-related chromatographic performances

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

5 years ago

Comparative study of strong cation exchangers: Structure-related chromatographic performances

Chromatographic performances are highly influenced by operational parameters. New ion exchangers have tailored matrices providing low backpressure, thereby allowing high flow velocity. By systematic frontal analysis and selectivity determination at different flow rates, we independently evaluated cation exchangers to facilitate media selection and investigated the relationship between surface modification and chromatographic performances. Structure-extended resins showed higher binding capacities compared to resins with conventional ligands directly attached to the matrix. Moreover, they maintained high capacities even with high flow velocities. Ligand accessibility was therefore largely enhanced, allowing proteins to interact and bind under harsh conditions with minimal residence/contact time. High throughput resins can be used for purification of high volume and high concentration feedstock in limited time. This results in higher productivity, and could contribute to cost reduction. In this work, we evaluated the dynamic binding capacities of various new ion exchange resins at different binding conductivities for different residence times, and observed that:

(i) Up to 7.5 mS/cm, Cellufine Max S-h and Max S-r have the highest dynamic binding capacity, which decreased with conductivity increasing beyond 10 mS/cm;

(ii) From 10 mS/cm, Nuvia S, GiGaCap S and Eshmuno S exhibit better dynamic binding capacity;

(iii) At higher conductivities, only Nuvia S shows a dynamic binding capacity of 100 mg/ml (at 12.5 mS/cm) and 80 mg/ml (at 15 mS/cm).

Publisher URL: www.sciencedirect.com/science

DOI: S1570023217316240