Waste Management and Research
Waste Management and Research
5 years ago

Recovery of Salmonella typhimurium from compost with the IMS–PCR method

Marco De Bertoldi, Giuseppe Damante, Francesca Venuti, Marcello Civilini
In this work a procedure was optimized to recover Salmonella typhimurium from a vegetable compost after its artificial contamination. A combination of a bacteriological method, immunomagnetic separation (IMS) and polymerase chain reaction (PCR) techniques allowed a reduction in the detection time to 30 h while maintaining high specificity. The lower threshold of direct amplification from extracts of contaminated compost was 108 salmonellae g−1. To improve the sensitivity, a combination of shortened pre-enrichment and enrichment procedures was optimized and the growth of S. typhimurium evaluated. Immunomagnetic separation using anti-Salmonella DynabeadsTM permitted the recovery of 30 salmonellae per 50 g of compost, the same value obtained with the traditional microbial method, which takes two days longer. Alternative purification methods to reduce organic compound inhibition of the PCR reaction mixture did not improve Salmonella detection under a threshold of 105 salmonellae g−1.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1034/j.1399-3070.2000.00160.x

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.