Journal of Clinical Periodontology
Journal of Clinical Periodontology
5 years ago

Antimicrobial peptide gene expression in periodontitis patients: a pilot study

L. Kanagaratnam, F. Velard, J. Sergheraert, S.C. Gangloff, L. Pierrard, J. Braux, B. Lefèvre, M.L. Jourdain
Aim Antimicrobial peptides (AMPs) are one of the most active components of innate immunity and have characteristics that could place them at the heart of the pathogenesis of periodontal disease. This study investigated differences in the expression of AMP coding genes obtained using a simple harvesting technique, gingival smear, between two groups of patients: chronic periodontitis subjects vs healthy ones. Materials and Methods Twenty-three patients were enrolled in two groups: 12 were diagnosed with moderate or severe generalized chronic periodontitis and 11 were diagnosed as clinically healthy. Gingival smears were retrieved and studied using reverse transcription quantitative PCR (RT-qPCR) after mRNA purification. Results Fifteen gene expressions were obtained using real-time RT-qPCR. Three AMP genes, Histatin 3 (HTN3), α-defensin 4 (DEFA4) and lysozyme C (LYZ), presented different expression levels in periodontitis patients compared to healthy subjects. The relative expression level of DEFA4 appeared to be a protective factor against periodontitis. Conclusion Gingival smears studied by RT-qPCR may be used to assess the expression of AMPs coding genes. A lack of expression of DEFA4 could be a potential indicator of periodontitis status. This article is protected by copyright. All rights reserved.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1111/jcpe.12879

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.