Development
Development
5 years ago

An evolutionarily conserved NIMA-related kinase directs rhizoid tip growth in the basal land plantMarchantia polymorpha.

Takayuki Kohchi, Taku Takahashi, Shogo Takatani, Ryuichi Nishihama, Kimitsune Ishizaki, Hiroyasu Motose, Kento Otani
Tip growth is driven by turgor pressure and mediated by the polarized accumulation of cellular materials. How a single polarized growth site is established and maintained is unclear. Here, we analyzed the function of NIMA-related protein kinase 1 (MpNEK1) in the liverwortMarchantia polymorphaIn the wild type, rhizoid cells differentiate from the ventral epidermis and elongate through tip growth to form hair-like protrusions. In Mpnek1knockout mutants, rhizoids underwent frequent changes in growth direction, resulting in a twisted and/or spiral morphology. The functional MpNEK1-Citrine protein fusion localized to microtubule foci in the apical growing region of rhizoids. Mpnek1knockouts exhibited increase in both microtubule density and bundling in the apical dome of rhizoids. Treatment with the microtubule-stabilizing drug taxol phenocopied Mpnek1knockout. These results suggest that MpNEK1 directs tip growth in rhizoids through microtubule organization. Furthermore, MpNEK1 expression rescued ectopic outgrowth of epidermal cells in theArabidopsis thaliana nek6mutant, strongly supporting an evolutionarily conserved NEK-dependent mechanism of directional growth. Such a mechanism may have contributed to the evolution of the early rooting system in land plants.

Publisher URL: http://doi.org/10.1242/dev.154617

DOI: 10.1242/dev.154617

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