Journal of Cell Science
Journal of Cell Science
5 years ago

The physiological regulation of macropinocytosis duringDictyosteliumgrowth and development.

Thomas D Williams, Robert R Kay
Macropinocytosis is a conserved endocytic process used byDictyosteliumamoebae for feeding on liquid medium. To furtherDictyosteliumas a model for macropinocytosis, we developed a high-throughput flow cytometry assay to measure macropinocytosis, and used it to identify inhibitors and investigate the physiological regulation of macropinocytosis.Dictyosteliumhas two feeding states: phagocytic and macropinocytic. When cells are switched from phagocytic growth on bacteria to liquid media, the rate of macropinocytosis slowly increases, due to increased size and frequency of macropinosomes. Upregulation is triggered by a minimal medium of 3 amino acids plus glucose and likely depends on macropinocytosis itself. The presence of bacteria suppresses macropinocytosis while their product, folate, partially suppresses upregulation of macropinocytosis. Starvation, which initiates development, does not of itself suppress macropinocytosis: this can continue in isolated cells, but is shut down by a conditioned-medium factor or activation of PKA signalling. Thus macropinocytosis is a facultative ability ofDictyosteliumcells, regulated by environmental conditions that are identified here.

Publisher URL: http://doi.org/10.1242/jcs.213736

DOI: 10.1242/jcs.213736

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