Nature Methods
Nature Methods
5 years ago

Conformational landscape of a virus by single-particle X-ray scattering

Conformational landscape of a virus by single-particle X-ray scattering
Reyhaneh Sepehr, Ali Dashti, Jeremy Copperman, Abbas Ourmazd, Brenda G Hogue, Chun Hong Yoon, Marius Schmidt, Russell Fung, Ahmad Hosseinizadeh, Garth J Williams, Ghoncheh Mashayekhi, Andrew Aquila, Peter Schwander
Using a manifold-based analysis of experimental diffraction snapshots from an X-ray free electron laser, we determine the three-dimensional structure and conformational landscape of the PR772 virus to a detector-limited resolution of 9 nm. Our results indicate that a single conformational coordinate controls reorganization of the genome, growth of a tubular structure from a portal vertex and release of the genome. These results demonstrate that single-particle X-ray scattering has the potential to shed light on key biological processes.

Publisher URL: http://dx.doi.org/10.1038/nmeth.4395

DOI: 10.1038/nmeth.4395

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