Clinical Microbiology and Infection
Clinical Microbiology and Infection
5 years ago

Dissemination of Panton-Valentine leukocidin-positive methicillin-resistant Staphylococcus aureus USA300 clone in multiple hospitals in Tokyo, Japan

Panton-Valentine leukocidin (PVL) gene (lukS/F-PV)-positive community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA), particularly the USA300 clone, is a health concern worldwide. Recently, CA-MRSA clones have been found to spread and persist in hospital settings. In this study, we aimed to investigate the prevalence and molecular epidemiological features of lukS/F-PV-positive MRSA in Japanese hospitals. Methods A total of 3,433 MRSA isolated from nine hospitals in 2011–2015 were used. Molecular epidemiological analysis performed by staphylococcal cassette chromosome (SCC) mec typing, PVL typing, arginine catabolic mobile element (ACME) typing, detection of virulence determinants, multilocus sequence typing, pulsed-field gel electrophoresis, and antimicrobial susceptibility testing. Result The detection rate of lukS/F-PV was increased annually from 0.6% (5/817) in 2011 to 3.1% (17/544) in 2015. Molecular epidemiological analysis for 64 lukS/F-PV-positive MRSA isolates revealed that 42 isolates (64.1%) were the USA300 clone. Resistance rates of levofloxacin and gentamicin among lukS/F-PV-positive isolates increased annually due to increased prevalence of the multidrug-resistant USA300 clone. Conclusion This is the first report on dissemination of the USA300 clone and PVL-positive MRSA in multiple Japanese hospitals. Our data strongly suggested that the USA300 clone may become epidemic in Japanese hospitals.

Publisher URL: www.sciencedirect.com/science

DOI: S1198743X1830185X

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