Robust Phenotypic Activation of Eosinophils during ExperimentalToxocara canisInfection.

5 years ago

Robust Phenotypic Activation of Eosinophils during ExperimentalToxocara canisInfection.

Débora Meira Neris, Luciana Camillo, Márcio Sobreira Silva Araújo, Fernanda de Freitas Anibal, Elaine Speziali, Andréa Teixeira-Carvalho, Jordana Grazziela Coelho-Dos-Reis, Joice Margareth de Almeida Rodolpho, Olindo Assis Martins-Filho, Ricardo de Oliveira Correia

Eosinophils are multifunctional cells that have cytotoxic proinflammatory activities and stimulate CD4⁺T-cells in experimental models of allergy and parasitic infections. Eosinophils, when exposed to antigens, are activated, expressing the CD38/CD69 molecules and exhibited increased expression of major histocompatibility complex (MHC-II), CD80 and CD86, suggesting they play a role uponToxocara canisantigen stimulation. In the present study, we evaluated the profile of eosinophils using conventional and image flow cytometry upon experimentalT. canisinfection.T. canisantigens induced a robust activation on this subset, contributing to the immune responses elicited in the experimental model forT. canis-associated visceral larva migrans syndrome. Data analysis demonstrated that, during murineT. canisinfection, eosinophils from peripheral blood, spleen, and bone marrow presented upregulated expression of CD69/MHC-II/CD80/CD86. As opposed to splenic and bone marrow eosinophils, circulating eosinophils had increased expression of activation markers uponT. canisinfection. The enhanced connectivity between eosinophils and T-cells inT. canis-infected mice in all three compartments (peripheral blood, spleen, and bone marrow) also supports the hypothesis that eosinophils may adopt a role duringT. canisinfection. Moreover,in vitro T. canisantigen stimulation resulted in activation and upregulation of co-stimulatory-related molecules by bone marrow-derived eosinophils. Our findings are evidence of activation and upregulation of important activation and co-stimulatory-related molecules in eosinophils and suggest a reshape of activation hierarchy toward eosinophils during experimentalT. canisinfection.

Publisher URL: http://doi.org/10.3389/fimmu.2018.00064

DOI: 10.3389/fimmu.2018.00064