Process Biochemistry
Process Biochemistry
5 years ago

l-Lactic acid production from liquefied cassava starch by thermotolerant Rhizopus microsporus: Characterization and optimization

l-Lactic acid production from liquefied cassava starch by thermotolerant Rhizopus microsporus: Characterization and optimization
The thermotolerant Rhizopus microsporus DMKU 33 capable of producing l-lactic acid from liquefied cassava starch was isolated and characterized for its phylogenetic relationship and growth temperature and pH ranges. The concentrations of (NH4)2SO4, KH2PO4, MgSO4 and ZnSO4·7H2O in the fermentation medium was optimized for lactic acid production from liquefied cassava starch by Rhizopus microsporus DMKU 33 in shake-flasks at 40°C. The fermentation was then studied in a stirred-tank bioreactor with aeration at 0.75vvm and agitation at 200rpm, achieving the highest lactic acid production of 84g/L with a yield of 0.84g/g at pH 5.5 in 3 days. Lactic acid production was further increased to 105–118g/L with a yield of 0.93g/g and productivity of 1.25g/L/h in fed-batch fermentation. R. microsporus DMKU 33 is thus advantageous to use in simultaneous saccharification and fermentation for l-lactic acid production from low-cost starchy substrates.

Publisher URL: www.sciencedirect.com/science

DOI: S1359511317308498

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