Clinical Microbiology and Infection
Clinical Microbiology and Infection
5 years ago

How to isolate, identify and determine antimicrobial susceptibility of anaerobic bacteria in routine laboratories?

There has been an increased interest in the study of anaerobic bacteria, which cause human infection during the past decade. Many new genera and species have been described by to use 16S rRNA gene sequencing of clinical isolates obtained from different infection sites with commercially available special culture media to support the growth of anaerobes. Several systems, such as anaerobic pouches, boxes, jars and chambers provide suitable anaerobic culture conditions to isolate even strict anaerobic bacteria successfully from clinical specimens. Beside the classical, time consuming identification methods and automated biochemical tests, the matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) has revolutionized identification of even unusual and slow growing anaerobes directly from culture plates, giving the possibility to provide timely information about anaerobic infections. Aims The aim of this review article is to present methods for routine laboratories, which carry out anaerobic diagnostics on different levels. Sources Relevant data from the literature mostly published during the last seven years are encompassed and discussed. Content The review involves topics on the anaerobes as members of commensal microbiota and they role causing infection, the key requirements for collection and transport of specimens, processing of specimens in the laboratory, incubation techniques, identification and antimicrobial susceptibility testing of anaerobic bacteria. Advantages, drawbacks and specific benefits of the methods are highlighted. Implications of the present review are to update and improve the anaerobic microbiology in laboratories with optimal conditions as well as to encourage its routine implementation in laboratories with restricted resources.

Publisher URL: www.sciencedirect.com/science

DOI: S1198743X18301812

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