Molecular Microbiology
Molecular Microbiology
5 years ago

Deinococcus radiodurans DR1088 is a novel RecF-interacting protein that stimulates single-stranded DNA annealing

Deinococcus radiodurans DR1088 is a novel RecF-interacting protein that stimulates single-stranded DNA annealing
Hong Xu, Kaiying Cheng, Ye Zhao, Guangzhi Xu, Yuejin Hua
RecF, together with the recombination mediators RecO and RecR, is required in the RecFOR homologous recombination repair pathway in bacteria. In this study, a recF-dr1088 operon, which is highly conserved in the Deinococcus-Thermus phylum, was identified in Deinococcus radiodurans. Interaction between DRRecF and DR1088 was confirmed by yeast two-hybrid and pull-down assays. DR1088 exhibited some RecO-like biochemical properties including single/double-stranded DNA binding activity, ssDNA binding protein (SSB) replacement ability and ssDNA (with or without SSB) annealing activity. However, unlike other recombination proteins, dr1088 is essential for cell viability. These results indicate that DR1088 might play a role in DNA replication and DNA repair processes. This article is protected by copyright. All rights reserved. A novel RecF-interacting protein, DR1088 was identified as an essential protein in Deinococcus radiodurans. This protein exhibits single/double-stranded DNA binding activity, ssDNA binding protein (SSB) replacement ability, and ssDNA (with or without SSB coated) annealing activity.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1111/mmi.13828

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