4 years ago

Simultaneous fluorescent detection of multiple metal ions based on the DNAzymes and graphene oxide

Simultaneous fluorescent detection of multiple metal ions based on the DNAzymes and graphene oxide
A novel fluorescent detection strategy for simultaneous detection of Cu2+, Pb2+ and Mg2+ based on DNAzyme branched junction structure with three kinds of DNAzymes and graphene oxide (GO) was presented. Three fluorophores labeled DNA sequences consisted with enzyme-strand (E-DNA) and substrate strand (S-DNA) were annealed to form DNAzyme branched junction structure. In the presence of target metal ion, the DNAzyme was activated to cleave the fluorophore labeled S-DNA. The S-DNA fragments were released and adsorbed onto GO surface to quench the fluorescent signal. The detection limit was calculated to be 1 nM for Cu2+, 200 nM for Mg2+, and 0.3 nM for Pb2+, respectively. This strategy was successfully used for simultaneous detection of Cu2+, Mg2+ and Pb2+ in human serum. Moreover, it had potential application for simultaneous detection of multiple metal ions in environmental and biological samples.

Publisher URL: www.sciencedirect.com/science

DOI: S000326701730819X

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.