Natural Product Research
Natural Product Research
5 years ago

RP-HPLC analysis of diterpene lactones in leaves and stem of different species of Andrographis

RP-HPLC analysis of diterpene lactones in leaves and stem of different species of Andrographis
Dayanand Dalawai, Chetan Aware, Jyoti P. Jadhav, Hosakatte Niranjana Murthy
In the present study diterpene lactones were quantified in leaves and stem of different species of Andrographis collected from Western Ghats of India using reverse phase high performance liquid chromatography (RP-HPLC) method. Different populations of AA (Andrographis alata), AE (Andrographis echioides), ALn (Andrographis lineata var. lineata), ALw (Andrographis lineata var. lawii), AM (Andrographis macrobotrys), AO (Andrographis ovata), AP (Andrographis paniculata), APr (Andrographis producta) and AS (Andrographis serphyllifolia) were assessed for the amount of AG (andrographolide), NAG (neoandrographolide) and DDAG (14-deoxy-11, 12-didehydroandrographolide) in leaves and stem. The most abundant diterpenoid was AG and highest amount of 68.35 mg/g DW was recorded in a population of AP. AG was also present in leaves of ALw at considerable level (40.85 mg/g DW). NAG was optimum in the leaves of AM (98.43 to 102.03 mg/g DW). DDAG was higher in the leaves of AP (16.01 mg/g DW).

Publisher URL: https://www.tandfonline.com/doi/full/10.1080/14786419.2019.1662004

Open URL: https://figshare.com/articles/RP-HPLC_analysis_of_diterpene_lactones_in_leaves_and_stem_of_different_species_of_i_Andrographis_i_/9785153/files/17544419.pdf

DOI: 10.1080/14786419.2019.1662004

Open access
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