4 years ago

Synthesis and Biological Evaluation of Ionizable Lipid Materials for the In Vivo Delivery of Messenger RNA to B Lymphocytes

Synthesis and Biological Evaluation of Ionizable Lipid Materials for the In Vivo Delivery of Messenger RNA to B Lymphocytes
Joseph R. Dorkin, Mark W. Tibbitt, Michael W. Heartlein, Kevin J. Kauffman, Daniel G. Anderson, Matthias A. Oberli, Eric A. Appel, Owen S. Fenton, Jung H. Yang, Frank DeRosa, James C. Kaczmarek, Robert Langer, Manhao D. Zeng, Rebecca L. McClellan, Faryal F. Mir, Siddharth Jhunjhunwala
B lymphocytes regulate several aspects of immunity including antibody production, cytokine secretion, and T-cell activation; moreover, B cell misregulation is implicated in autoimmune disorders and cancers such as multiple sclerosis and non-Hodgkin's lymphomas. The delivery of messenger RNA (mRNA) into B cells can be used to modulate and study these biological functions by means of inducing functional protein expression in a dose-dependent and time-controlled manner. However, current in vivo mRNA delivery systems fail to transfect B lymphocytes and instead primarily target hepatocytes and dendritic cells. Here, the design, synthesis, and biological evaluation of a lipid nanoparticle (LNP) system that can encapsulate mRNA, navigate to the spleen, transfect B lymphocytes, and induce more than 60 pg of protein expression per million B cells within the spleen is described. Importantly, this LNP induces more than 85% of total protein production in the spleen, despite LNPs being observed transiently in the liver and other organs. These results demonstrate that LNP composition alone can be used to modulate the site of protein induction in vivo, highlighting the critical importance of designing and synthesizing new nanomaterials for nucleic acid delivery. B-lymphocytesregulate several aspects of immunity, but B-cell dysfunction can also serve as the root of disease. Accordingly, it is important to develop chemical tools that can modulate pathways associated with B-cell function. A new class of ionizable lipid materials is synthesized that, when formulated into messenger RNA lipid nanoparticles, induces protein expression within B-lymphocytes in mice.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/adma.201606944

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