3 years ago

Enhanced production of glutarate by using anaerobic-aerobic shift cultivation and an anaerobically inducible promoter in an engineered Escherichia coli

Enhanced production of glutarate by using anaerobic-aerobic shift cultivation and an anaerobically inducible promoter in an engineered Escherichia coli
Recently we constructed a recombinant Escherichia coli for glutarate production using α-ketoacids reduction pathway, in which the central carbon metabolite α-ketoglutarate is reduced to 2-hydroxyglutarate then converted to glutarate. However, the production titer of glutarate was low, which may be due to the oxygen-sensitive nature of 2-hydroxyglutaryl-CoA dehydratase (HgdABC) while the anaerobic cell metabolism is not so active. To achieve higher production of glutarate, an aerobic-anaerobic shift strategy was developed by fast growing cells aerobically, followed by a shift to anaerobic cultivation to ensure the activity of HgdABC for glutarate biosynthesis. The two-stage cultivation strategy resulted in approximately 30% higher production titer than the original anaerobic cultivation. Furthermore, an anaerobically-inducible nar promoter was employed to control the hgdABC expression, and the newly engineered strain showed efficient expression of hgdABC responding to aerobic-anaerobic shift during two-stage cultivation. Finally, the glutarate production titer was doubled, reaching 11.6mg/L. The work demonstarted the potential of the integration of bioprocess engineering and metabolic engineering for enhancing bulk chemicals production by an engineered microorganism.

Publisher URL: www.sciencedirect.com/science

DOI: S1359511317309625

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.