3 years ago

Gene expression of selenoproteins can be regulated by thioredoxin(Txn) silence in chicken Cardiomyocytes

Gene expression of selenoproteins can be regulated by thioredoxin(Txn) silence in chicken Cardiomyocytes
Thioredoxin (Txn) system is the most crucial antioxidant defense mechanism in myocardium. Hamper in Txn system may compromise cell survival through oxidative stress induction. The aim of this present study was to clarify the effect of Txn low expression in chicken cardiomyocytes for 25 selenoproteins. We developed a Se-deficient induced myocardial injury model (0.033mg/kg) for in-vivo and Txn knock down cardiomyocytes culture model (siRNA) for in-vitro studies. Western blot, qPCR were performed, and correlation analysis, heat map were used for further analysis. The mRNA and protein levels of the Txn were significantly decreased (P<0.05) in both of models, which demonstrates successful model creation for Txn low expressions. Both low expression of Txn models are significantly decreased (P<0.05) the mRNA levels of Dio1, Dio2, Gpx1, Gpx2, Gpx3, Gpx4, TR1, TR2, TR3, Selt, Selw, SelK, Sepx1, and significantly increased (P<0.05) the mRNA levels of the rest of selenoproteins. Correlation analysis showed that Dio3, Selm, Selp15, Selh, Selu, Seli, Seln, Sepp1, SelO, Sels, SelS2 and Selpb had a negative correlation with Txn, while the rest of selenoproteins had a positive correlation with Txn. Combine in vivo and in vitro we can know that hamper Txn expression can inhibit glutathione peroxidase (Gpx1, 2, 3, 4), thioredoxin reductase (TR1, 2, 3) and deiodinase (Dio1, 2), Selt, Selw, SelK, Sepx1, meanwhile, over expression the rest of selenoproteins. In conclusion, that different selenoproteins possess and exhibits distinct responses to silence of Txn in chicken cardiomyocytes.

Publisher URL: www.sciencedirect.com/science

DOI: S0162013417304257

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