3 years ago

Kinetics of cellulose hydrolysis by halostable cellulase from a marine Aspergillus niger at different salinities

Kinetics of cellulose hydrolysis by halostable cellulase from a marine Aspergillus niger at different salinities
Kinetics of cellulose hydrolysis with halostable cellulase from a marine Aspergillus niger was analyzed at different salinities. Cellulase activity in 8% NaCl solution was 1.43 folds higher than that in NaCl free solution. Half saturation constant, Km (15.6260g/L) and the rate constant of deactivation, Kde (0.3369g/Lh) in 8% NaCl solution was lower than that (18.6364g/L), 0.3754 (g/Lh) in NaCl free solution. The maximum initial hydrolysis velocity, Vmax (25.5295g/Lh), in 8% NaCl solution was higher than that in NaCl free solution (25.0153 (g/Lh). High salinity increased affinity to the cellulase to the substrate and thermostability. Halostable cellulase from a marine Aspergillus niger was valuable for cellulose hydrolysis under high salinity conditions.

Publisher URL: www.sciencedirect.com/science

DOI: S1359511317309777

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