4 years ago

Terbium(III) Luminescent Complexes as Millisecond-Scale Viscosity Probes for Lifetime Imaging

Terbium(III) Luminescent Complexes as Millisecond-Scale Viscosity Probes for Lifetime Imaging
Anh Thy Bui, Olivier Maury, Alexei Grichine, Alain Duperray, Chantal Andraud, Pierre Lidon, François Riobé
Fluorescent probes that are able to directly measure viscosity are attractive candidates for the study of intracellular environments. We report a new class of luminescent rotors, based on the sensitized emission of a terbium(III) complex. A 4-fold increase in both quantum yield and luminescence lifetime was observed in viscous media for the studied complexes, with a lifetime ranging from 0.23 to 0.89 ms over a broad range of viscosities (0.6–1200 cP). The presented approach, relying on the millisecond-scale luminescence lifetime of the lanthanide ions, was applied to fixed T24 cancer cells using temporal sampling lifetime imaging microscopy.

Publisher URL: http://dx.doi.org/10.1021/jacs.7b02951

DOI: 10.1021/jacs.7b02951

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