Investigation of the mechanism of the SpnF-catalyzed [4+2]-cycloaddition reaction in the biosynthesis of spinosyn A [Chemistry]
The Diels–Alder reaction is one of the most common methods to chemically synthesize a six-membered carbocycle. While it has
long been speculated that the cyclohexene moiety found in many secondary metabolites is also introduced via similar chemistry,
the enzyme SpnF involved in the biosynthesis of the insecticide spinosyn A in Saccharopolyspora spinosa is the first enzyme for which catalysis of an intramolecular
[4+2]-cycloaddition has been experimentally verified as its only known function. Since its discovery, a number of additional standalone
[4+2]-cyclases have been reported as potential Diels–Alderases; however, whether their catalytic cycles involve a concerted or
stepwise cyclization mechanism has not been addressed experimentally. Here, we report direct experimental interrogation of
the reaction coordinate for the
[4+2]-carbocyclase SpnF via the measurement of
α-secondary deuterium kinetic isotope effects (KIEs) at all sites of
sp2→sp3 rehybridization for both the nonenzymatic and enzyme-catalyzed cyclization of the SpnF substrate. The measured KIEs for the
nonenzymatic reaction are consistent with previous computational results implicating an intermediary state between formation
of the first and second carbon–carbon bonds. The KIEs measured for the enzymatic reaction suggest a similar mechanism of cyclization
within the enzyme active site; however, there is evidence that conformational restriction of the substrate may play a role
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