3 years ago

Development of a Vivid FRET System Based on a Highly Emissive dG–dC Analogue Pair

Development of a Vivid FRET System Based on a Highly Emissive dG–dC Analogue Pair
Ji Hoon Han, Soyoung Park, Seigi Yamamoto, Hiroshi Sugiyama
A new type of Förster Resonance Energy Transfer (FRET) system using highly emissive isomorphic nucleobase analogues is reported. The FRET pair consists of 2-aminothieno[3,4-d]pyrimidine G-mimic deoxyribonucleoside (thdG) as an energy donor and 1,3-diaza-2-oxophenothiazine (tC) as an energy acceptor. The distance and orientation between donor and acceptor was controlled by systematic incorporation of thdG and tC into DNA sequences to investigate the FRET efficiencies. This is the first Watson–Crick base-pairable FRET pair to produce vivid colors. In addition, this nucleic acid-based FRET pair was used to monitor DNA conformation and achieved visualization of the B–Z transition. A new type of FRET system using highly emissive isomorphic nucleobase analogues is reported. The FRET pair consists of 2-aminothieno[3,4-d]pyrimidine G-mimic deoxyribonucleoside (thdG) as an energy donor and 1,3-diaza-2-oxophenothiazine (tC) as an energy acceptor. The distance and orientation between donor and acceptor was controlled to investigate the FRET efficiencies. This is the first Watson–Crick base-pairable FRET pair to produce vivid colors. In addition, this nucleic acid-based FRET pair was used to monitor DNA conformation and achieved visualization of the B–Z transition.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/chem.201701118

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