European Respiratory Journal
European Respiratory Journal
4 years ago

Analysis of microRNA expression profile in adipose tissue during allergic inflammation in the rat

Dawid Szczepankiewicz
An increased amount of adipose tissue enhances airway inflammation, but the mechanism how the adipose tissue communicates with a physically distant respiratory system is unknown. A possible mediator between adipose tissue and airways can be miRNAs packed in extracellular vesicles released by various types of tissues. In this study, we aimed to profile microRNAs isolated from adipose tissue and from exosomes in bronchoalveolar lavage in rat model of alelrgy. Brown Norway rats were sensitized to house dust mite (HDM) was used, administered first subcutaneously and then intranasal to the upper respiratory tract. To confirm the induction of allergic inflammation, the eosinophil count and the total IgE level were determined. For miRNA expression profiling, the next generation sequencing method was used. The presence of inflammation was confirmed in sensitized rats (elevated concentrations of both eosinophils and total IgE). Differential miRNA expression between HDM-sensitized and control rats showed significant differences in the expression of 40 miRNA genes from the pool of 1835 genes expressed in adipose tissue. The altered expression of miRNAs in adipose tissue of allergic animals (miR-375-3p, miR-193a-3p) was confirmed in exosomes from bronchoalveolar lavage fluid (BAL) suggesting their role in airway inflammation. The study was supported by the Polish National Science Centre, grant no. 2017/01/X/NZ4/00467 (D. Szczepankiewicz)

Publisher URL: http://erj.ersjournals.com/cgi/content/short/54/suppl_63/PA5407

DOI: 10.1183/13993003.congress-2019.PA5407

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