5 years ago

Cancer Cell Discrimination Using Host–Guest “Doubled” Arrays

Cancer Cell Discrimination Using Host–Guest “Doubled” Arrays
Subinoy Rana, Ngoc D. B. Le, Mahdieh Yazdani, D. Joseph Jerry, Gulen Yesilbag Tonga, Rajesh Ramanathan, Vincent M. Rotello, Rubul Mout, Sung-Tae Kim, Marcos E. Wille, Karen A. Dunphy, Caren M. Rotello
We report a nanosensor that uses cell lysates to rapidly profile the tumorigenicity of cancer cells. This sensing platform uses host–guest interactions between cucurbit[7]uril and the cationic headgroup of a gold nanoparticle to non-covalently modify the binding of three fluorescent proteins of a multi-channel sensor in situ. This approach doubles the number of output channels to six, providing single-well identification of cell lysates with 100% accuracy. Significantly, this classification could be extended beyond the training set, determining the invasiveness of novel cell lines. The unique fingerprint of these cell lysates required minimal sample quantity (200 ng, ∼1000 cells), making the methodology compatible with microbiopsy technology.

Publisher URL: http://dx.doi.org/10.1021/jacs.7b03657

DOI: 10.1021/jacs.7b03657

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