5 years ago

Transcriptomic analysis of gingival mesenchymal stem cells cultured on 3D bioprinted scaffold: A promising strategy for neuroregeneration

Oriana Trubiani, Paolo Cardelli, Agnese Gugliandolo, Francesca Diomede, Emanuela Mazzon, Placido Bramanti, Domenico Scionti, Alessia Bramanti
The combined approach of Mesenchymal stem cells (MSCs) and scaffolds has been proposed as a potential therapeutic tool for the treatment of neurodegenerative diseases. Indeed, even if MSCs can promote neuronal regeneration, replacing lost neurons or secreting neurotrophic factors, many limitations still exist for their application in regenerative medicine, including the low survival and differentiation rate. The scaffolds, by mimicking the endogenous microenvironment, have shown to promote cell survival, proliferation and differentiation. In this work gingival mesenchymal stem cells (GMSCs), isolated from healthy donors, were expanded in vitro, by culturing them adherent in plastic dishes (CTR-GMSCs) or on a poly-(lactic acid) scaffold (SC-GMSCS). In order to evaluate the survival and the neurogenic differentiation potential we performed a comparative transcriptomic analysis between CTR-GMSCs and SC-GMSCs by Next Generation Sequencing (NGS). We found that SC-GMSCs showed an increased expression of neurogenic and pro-survival genes. In particular, genes involved in neurotrophin signaling and PI3K/Akt pathways were upregulated. On the contrary, pro-apoptotic and negative regulator of neuronal growth genes were downregulated. Moreover, nestin and GAP-43 protein levels increased in SC-GMSCs, confirming the neurogenic commitment of these cells. In conclusion, the scaffold, providing a trophic support for MSCs, may promote GMSCs differentiation toward a neuronal phenotype and survival. This article is protected by copyright. All rights reserved.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/jbm.a.36213

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