5 years ago

Cross-linked basement membrane based coatings enhance glucose sensor function and continuous glucose monitoring in vivo

Ulrike Klueh, Donald L. Kreutzer, Yi Qiao, Caroline Czajkowski, Izabela Ludzinska
Overcoming sensor induced tissue reactions is an essential element of achieving successful continuous glucose monitoring (CGM) in the management of diabetes, particularly when used in closed loop technology. Recently, we demonstrated that basement membrane (BM) based glucose sensor coatings significantly reduced tissue reactions at sites of device implantation. However, the biocompatible BM based bio-hydrogel sensor coating rapidly degraded over a less than a three-week period, which effectively eliminated the protective sensor coating. In an effort to increase the stability and effectiveness of the BM coating, we evaluated the impact of cross-linking BM utilizing glutaraldehyde as a cross-linking agent, designated as X-Cultrex. Sensor performance (non-recalibrated) was evaluated for the impact of these X-Cultrex coatings in vitro and in vivo. Sensor performance was assessed over a 28-day time period in a murine CGM model and expressed as mean absolute relative difference (MARD) values. Tissue reactivity of Cultrex coated, X-Cultrex coated, and uncoated glucose sensors was evaluated over a 28-day time period in vivo using standard histological techniques. These studies demonstrated that X-Cultrex based sensor coatings had no effect on glucose sensor function in vitro. In vivo, glucose sensor performance was significantly enhanced following X-Cultrex coating throughout the 28-day study. Histological evaluations of X-Cultrex treated sensors demonstrated significantly less tissue reactivity when compared to uncoated sensors. This article is protected by copyright. All rights reserved.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/jbm.a.36206

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