3 years ago

Rare coding variants in MAPK7 predispose to adolescent idiopathic scoliosis

Rare coding variants in MAPK7 predispose to adolescent idiopathic scoliosis
Yubin Deng, Xianjian Qiu, Junlin Yang, Chiea Chuen Khor, Youqiang Song, Bo Gao, Chong Chen, Yulan Chen, Wentao Liu, Taifeng Zhou, Wenjie Gao, Chao Xing, Dingjun Hao, Silong Sun, Kenneth M. C. Cheung, Pak Chung Sham, Chengjie Lian, Keith K. D. Luk, Deying Su, Esam Alattar, Shulan Yang, Caixia Xu, Xiaoming Yang, Quan-Zhen Li, Peiqiang Su, Danny Chan, Gabriel Liu, Zizhao Wu, Dongsheng Huang, Hang Zhou
Adolescent idiopathic scoliosis (AIS) is a complex genetic disorder characterized by three-dimensional spinal curvatures, affecting 2%–3% of school age children, yet the causes underlying AIS are not well understood. Here, we first conducted a whole-exome sequencing and linkage analysis on a three-generation Chinese family with autosomal-dominant (AD) AIS, and then performed targeted sequencing in a discovery cohort comprising 20 AD AIS families and 86 simplex patients, and finally identified three disease-associated missense variants (c.886G> A, c.1943C> T, and c.1760C> T) in the MAPK7 gene (encoding mitogen-activated protein kinase 7). Genotyping of the three rare variants in a Chinese replication cohort comprising 1,038 simplex patients and 1,841 controls showed that their combined allele frequency was significantly over-represented in patients as compared with controls (2.0% [41/2,076] vs. 0.7% [27/3,682]; odds ratio = 2.7; P = 2.8 × 10−5). In vitro, we demonstrated that the three MAPK7 mutants disrupted nuclear translocation in cellular models, which is necessary for the normal function of MAPK7. In vivo, we also conducted CRISPR/Cas9-mediated deletion of mapk7 in zebrafish recapitulating the characteristic phenotype of idiopathic scoliosis. Taken together, our findings suggest that rare coding variants in MAPK7 predispose to AIS, providing clues to understanding the mechanisms of AIS. We conducted a whole-exome sequencing and linkage analysis on a three-generation Chinese family with autosomal dominant (AD) AIS, then performed targeted sequencing in a discovery cohort, and finally identified three disease-associated missense variants (c.886G>A, c.1943C>T and c.1760C>T) in the MAPK7 gene. In vitro, we demonstrated that the three MAPK7 mutants disrupted nuclear translocation in cellular models. In vivo, we also conducted CRISPR/Cas9-mediated deletion of mapk7 in zebrafish recapitulating the characteristic phenotype of idiopathic scoliosis.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/humu.23296

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