Immunology
Immunology
5 years ago

1,25-dihydroxyvitamin D3-induced dendritic cells suppress experimental autoimmune encephalomyelitis by increasing proportions of the regulatory lymphocytes and reducing T helper type 1 and type 17 cells

1,25-dihydroxyvitamin D3-induced dendritic cells suppress experimental autoimmune encephalomyelitis by increasing proportions of the regulatory lymphocytes and reducing T helper type 1 and type 17 cells
Jing Wu, Zhongxiang Xie, Jingtao Chen, Chenhong Lin, Chao Zheng, Jie Zhu, Tao Jin, Shan Zhu, Yun Cheng, Qingqing Cao
Dendritic cells (DCs), a bridge for innate and adaptive immune responses, play a key role in the development of multiple sclerosis (MS) and experimental autoimmune encephalomyelitis (EAE), an animal model for MS. Administration of tolerogenic DCs has been used as an immunotherapy in autoimmune diseases. Deficiency of vitamin D is an environmental risk factor of MS. In this study, we induced tolerogenic DCs by 1,25-dihydroxyvitamin D3 and transferred the tolerogenic DCs (VD3-DCs) into EAE mice by adoptive transfer. We found that VD3-DCs inhibited the infiltrations of T helper type 1 (Th1) and Th17 cells into spinal cord and increased the proportions of regulatory T cells (CD4+ CD25+ Foxp3+), CD4+ IL-10+ T cells and regulatory B cells (CD19+ CD5+ CD1d+) in peripheral immune organs, which resulted in attenuated EAE. However, the proportions of T helper type 1 (Th1) and Th17 cells in spleen and lymph nodes and the levels of pro-inflammatory cytokines and IgG in serum also increased after transfer of VD3-DCs. We conclude that transfer of VD3-DCs suppressed EAE by increasing proportions of regulatory T cells, CD4+ IL-10+ T cells and regulatory B cells in spleen and reducing infiltration of Th1 and Th17 cells into spinal cord, which suggests a possible immunotherapy method using VD3-DCs in MS. 1,25-Dihydroxyvitamin D3 induced tolerogenic DCs (VD3-DCs) showed a similar characterization with tolerogenic DCs. Adoptive transfer of VD3-DCs suppressed experimental autoimmune encephalomyelitis by increasing proportions of regulatory T cells, CD4+ IL-10+ T cells and regulatory B cells in spleen and reducing infiltration of Th1 and Th17 cells into spinal cord.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1111/imm.12776

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