3 years ago

Pru p 3-Epitope-based sublingual immunotherapy in a murine model for the treatment of peach allergy

Pru p 3-Epitope-based sublingual immunotherapy in a murine model for the treatment of peach allergy
Maria J. Torres, Araceli Diaz-Perales, Javier Rojo, Nuria Cubells-Baeza, Francisca Gomez, Cristobalina Mayorga, Ainhoa Mascaraque, Maria J. Rodriguez, James R. Perkins, Javier Ramos-Soriano, David Andreu, Maria Garrido-Arandia
Scope Food-specific immunotherapy (SIT) is a promising treatment for lipid transfer protein (LTP)-syndrome. We propose a novel sublingual-SIT (SLIT) that combines a Pru p 3 T-cell peptide and an oligodeoxyribonucleotide (ODN) with CpG motifs (ODN-CpG) as adjuvants to induce a specific Th1/Treg response. Methods and results LTP-peach allergic mice were treated sublingually with a combination of a CpG sequence and mono- or tetravalent systems including a Pru p 3 peptide, D1(Prup3) or D4(Prup3). Mice were challenged intraperitoneally with Pru p 3 one or three weeks after SLIT and tolerance was assessed. Mice treated with D1(Prup3)+CpG were protected from anaphylaxis after Pru p 3 challenge. They showed no change in body temperature, lower levels of Pru p 3-specific IgE and IgG1 antibodies and higher levels of sIgG2a compared to the untreated group. They had fewer IgE and IgG1 secreting cells and more sIgG2a secreting cells. Moreover, a significantly lower number of Pru p 3-specific CD4+T cells and a higher number of Treg cells were found, alongside a Th1 cytokine pattern. These changes were maintained for three weeks after stopping treatment. Conclusion D1Prup3+CpG represents a promising SIT for food allergy. It is easily synthesized and induces protection from anaphylaxis to Pru p 3 that is maintained for at least three weeks. In this study, LTP-peach anaphylactic mice are treated sublingually with a combination of a CpG sequence and mono- or tetravalent systems including a Pru p 3 peptide. It is found that the monomeric compound administered sublingually represents a promising specific immunotherapy for food allergy since it is easily synthesized, safe, and induces a persistent protection from anaphylaxis to Pru p 3.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/mnfr.201700110

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