4 years ago

Functional Polysaccharide Sutures Prepared by Wet Fusion of Interfacial Polyelectrolyte Complexation Fibers

Functional Polysaccharide Sutures Prepared by Wet Fusion of Interfacial Polyelectrolyte Complexation Fibers
Joseph P. Park, Jae-Hyung Jang, Byung Gee Im, Haeshin Lee, Minjae Do
This study reports polysaccharide-based fibers that can be utilized as biocompatible functional sutures. Fibers are spontaneously formed by spinning at the interface between two oppositely charged polysaccharide solutions. Unlike the common belief that polysaccharide fibers prepared by electrostatic interactions would exhibit weak mechanical strength, it is demonstrated that fibers spun at the interface between two droplets of positively charged chitosan and negatively charged heparin can exhibit high mechanical strength through spontaneous wet-state fusion of interfiber strands at a spinning wheel. Dry solidification results in multistranded fibers that were ≈100 µm in diameter with a tensile strength of ≈220 MPa. Post fibrous manipulation yields various morphology with straight or twisted fibers, fabrics, or springs. To demonstrate application of the fiber, it is applied as a medical suture. As heparin has a unique ability to bind adeno-associated virus (AAV), a therapeutic, biocompatible suture exhibiting localized AAV-mediated gene delivery function can be prepared. This study shows that multistrand fusion of fibers, formed by weak, electrostatic interactions and followed by drying solidification counterintuitively results in mechanically strong, functional fibers with various potential applications. The adeno-associated virus (AAV) immobilized chitosan/heparin suture is fabricated for localized gene delivery. Implantation of the AAV immobilized chitosan/heparin suture in subcutaneous tissue results in highly localized fluorescence signal expression at the implant site in the timeframe from three to five weeks. The developed chitosan/heparin suture holds great promise for clinical gene delivery.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/adfm.201702017

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