5 years ago

Live cell imaging of bacterial cells: Pyrenoylpyrrole-based fluorescence labeling

Live cell imaging of bacterial cells: Pyrenoylpyrrole-based fluorescence labeling
Sivakumar Shanmugam, Mathiyazhagan Arun Divakar
A novel substituted pyrenoylpyrroles was synthesized by the reaction of pyrenoyl chalcone, TosMIC and methyl iodide under mild condition. All the synthesized compounds were screened for their bioactivity, and the MIC was determined, among which few compounds showed moderate antibacterial activity toward Gram-positive as well as Gram-negative bacteria. Further, cytotoxicity assay ascertained that these compounds were non-toxic to mammalian cells as well. The pyrene chromophore in the synthesized compounds (3a–e) and (5a–e) is responsible for the good photophysical properties which have an absorbance at λ 340 nm and emission at λ 410 nm. Hence, two of the selected novel synthesized compounds with non-cytotoxic nature prospected for bio-imaging of bacterial cells using high-content screening analysis show that the molecule is suitable for microbial imaging in pathological diagnostic studies. Novel pyrenoylpyrrole compounds were synthesized from easily available synthons. All the compounds were non-cytotoxic, potential candidates for microbial cell imaging to aid diagnostics with moderate antibacterial activity.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1111/cbdd.12978

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