4 years ago

A neutralization assay for Zika and Dengue viruses using a real-time PCR-based endpoint assessment.

Dupont-Rouzeyrol M, Tran T, Catton M, Druce J, Wilson HL
The global spread and infective complications of Zika virus (ZKV) and Dengue virus (DENV) have made them flaviviruses of public health concern. Serological diagnosis can be challenging due to antibody cross-reactivity, particularly in secondary flavivirus infections or when there is a history of flavivirus vaccination. The virus neutralization assay is considered to be the most specific assay for measurement of anti-flavivirus antibodies. This study describes an assay where neutralization endpoint is measured by real-time PCR, providing results within 72 hours. It demonstrated 100% sensitivity (24/24 ZKV and 15/15 DENV) and 100% specificity (11/11 specimens) when testing well-characterised sera. In addition, the assay was able to determine the correct DENV serotype in 91.7% of cases. The high sensitivity and specificity of the real-time PCR neutralization assay makes it suitable to use as a confirmatory test for sera that are reactive in commercial IgM/IgG enzyme immunoassays. Results are objective and the PCR-based measurement of neutralisation endpoint lends itself to automation so that throughput may be increased in times of high demand.

Publisher URL: https://www.ncbi.nlm.nih.gov/pubmed/28794181

DOI: PubMed:28794181

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