3 years ago

Detection of Plasmodium spp. infection by the illumigene® Malaria assay compared to reference microscopy and real-time PCR.

Chan W, Chow B, Pillai DR, Church D, Rypien C
Malaria is one of the leading causes of infectious disease in returning travelers from the tropics. Diagnosis of malaria is typically performed by examination of Giemsa-stained thick and thin peripheral blood smears, which is time-consuming, labor-intensive and requires high levels of proficiency. Alternatively, loop-mediated isothermal amplification (LAMP) is a new molecular method, which is rapid, sensitive, and requires less capital equipment and technological training. We conducted a retrospective study comparing two formats of a commercial LAMP assay (Meridian illumigene® Malaria [M] and Malaria Plus [MP]) versus reference microscopy on archived blood specimens (n=140) obtained from unique returning travelers suspected of having malaria. Discrepant results were resolved by either repeat testing or a laboratory developed ultra-sensitive real-time PCR method. On initial testing, the Meridian illumigene® M and MP kits had a sensitivity of 97.3% [90.7 - 99.7%]and 100.0% [95.1- 100.0%] and specificity of 93.8% [95% CI; 84.8 - 98.3%] and 91.5% [81.3 - 97.2%], respectively, versus reference microscopy. We project a significant cost reduction in low prevalence non-endemic settings with LAMP-based malaria screening given the excellent negative predictive value achieved with LAMP compared to traditional rapid malaria tests.

Publisher URL: https://www.ncbi.nlm.nih.gov/pubmed/28768730

DOI: PubMed:28768730

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.