3 years ago

Investigation of Respiratory Syncytial Virus Outbreak on an Adult Stem Cell Transplant Unit using Whole Genome Sequencing.

Qi C, Green SJ, Lei Z, Metzger KE, Zhu Y, Zembower TR
A viral whole genome sequencing strategy, based on PCR amplification followed by Next-generation Sequencing, was used to investigate a nosocomial RSV-B outbreak in a hematology-oncology and stem cell transplant unit. RSV-B genomes from 16 patients and healthcare workers (HCWs) suspected to be involved in the outbreak were compared to RSV-B genomes acquired from outpatients during the same time period but epidemiologically unrelated to the outbreak. Phylogenetic analysis of the whole genome identified a cluster of 11 patients and HCWs with an identical RSV-B strain which were clearly distinct from strains recovered from individuals unrelated to the outbreak. Sequences variation of the G gene alone was insufficient to distinguish the outbreak strains from the outbreak unrelated strains, thereby demonstrating that WGS is valuable for local outbreak investigation.

Publisher URL: https://www.ncbi.nlm.nih.gov/pubmed/28747373

DOI: PubMed:28747373

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