Infection and Immunity
Infection and Immunity
5 years ago

Th17-mediated cross protection against pneumococcal carriage by vaccination with a variable antigen.

Pupo E, Houben D, Azarian T, Kuipers K, Langereis JD, Simonetti E, Jong WSP, Zomer A, Luirink J, van Selm S, van Opzeeland F, van der Gaast-de Jongh CE, van der Ley P, de Groot R, de Jonge MI, Koenders MI
Serotype-specific protection against a Streptococcus pneumoniae is an important limitation of the current polysaccharide-based vaccines. To prevent serotype replacement, reduce transmission and limit emergence of new variants, it is essential to induce broad protection and restrict pneumococcal colonization. In this study, we used a prototype vaccine formulation consisting of LPS-detoxified outer membrane vesicles (OMVs) from Salmonella Typhimurium displaying the variable N-terminus of PspA (α1α2) for intranasal vaccination, which induced strong Th17 immunity associated with substantial reduction of pneumococcal colonization. Despite the variable nature of this protein a common MHCII epitope was identified, based on in silico prediction combined with ex vivo screening, and was essential for the IL17A-mediated cross-reactivity and associated with cross-protection. Based on 1352 PspA sequences derived from a pneumococcal carriage cohort, this OMV-based vaccine formulation containing a single α1α2 type was estimated to cover 19.1% strains, illustrating the potential of Th17-mediated cross protection.

Publisher URL: https://www.ncbi.nlm.nih.gov/pubmed/28717032

DOI: PubMed:28717032

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.