3 years ago

cAMP signaling primes lung endothelial cells to activate caspase-1 during Pseudomonas aeruginosa infection

Phoibe Renema, Kierra S. Hardy, Nicole Housley, Grace Dunbar, Naga Annamdevula, Andrea L Britain, Domenico Spadafora, Silas Leavesley, Thomas C Rich, Jonathon P. Audia, Diego F. Alvarez
Activation of the inflammasome-caspase-1 axis in lung endothelial cells is emerging as a novel arm of the innate immune response to pneumonia and sepsis caused by Pseudomonas aeruginosa. Increased levels of circulating autacoids are hallmarks of pneumonia and sepsis, and induce physiological responses via cAMP signaling in targeted cells. However, it is unknown whether cAMP affects other functions such as P. aeruginosa-induced caspase-1 activation. Herein, we describe the effects of cAMP signaling on caspase-1 activation using a single cell flow cytometry-based assay. P. aeruginosa infection of cultured lung endothelial cells caused caspase-1 activation in a distinct population of cells. Unexpectedly, pharmacological cAMP elevation increased the total number lung endothelial cells with activated caspase-1. Interestingly, the addition of cAMP agonists augmented P. aeruginosa infection of lung endothelial cells as a partial explanation underlying cAMP priming of caspase-1 activation. The cAMP effect(s) appeared to function as a priming signal, because the addition of cAMP agonists was required either prior to, or early during the onset of infection. However, absolute cAMP levels measured by ELISA were not predictive of cAMP priming effects. Importantly, inhibition of de novo cAMP synthesis decreased the number of lung endothelial cells with activated caspase-1 during infection. Collectively, our data suggest lung endothelial cells rely on cAMP signaling to prime caspase-1 activation during P. aeruginosa infection.

Publisher URL: https://journals.physiology.org/doi/abs/10.1152/ajplung.00185.2019

DOI: 10.1152/ajplung.00185.2019

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