Biotransformation of 5‐Hydroxymethylfurfural to 2,5‐Furandicarboxylic Acid by a Syntrophic Consortium of Engineered Synechococcus Elongatus and Pseudomonas Putida
2,5‐furandicarboxylic acid (FDCA) is one of the top platform chemicals that can be produced from biomass feedstock. To make the cost of industrial FDCA production compatible with plastics made from fossils, the price of substrates and process complexity should be reduced. The aim of this research was to create a CO2‐driven syntrophic consortium for the catalytic conversion of renewable biomass‐derived 5‐hydroxymethylfurfural (HMF) to FDCA. Sucrose produced from carbon fixation by the engineered Synechococcus elongatus served as the sole carbon source for the engineered Pseudomonas putida to catalyze the reaction of HMF to FDCA. The yield of FDCA by the consortium reached around 70% while the conversion of HMF was close to 100%. With further surface engineering to clump the two strains, the FDCA yield was elevated to almost 100% via the specific association between an SH3 domain and its ligand. The syntrophic consortium successfully demonstrated its green and cost‐effective characteristics for the conversion of CO2 and biomass into platform chemicals.
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