3 years ago

2D Diffusion‐Ordered 1H‐NMR Spectroscopy Lipidomic Profiling After Oral Single Macronutrient Loads: Influence of Obesity, Sex, and Female Androgen Excess

María Insenser, Samuel Moncayo, M. Ángeles Martínez-García, Elena Fernández-Durán, Sara Samino, Francisco Álvarez-Blasco, Manuel Luque-Ramírez, Héctor F. Escobar-Morreale

Scope: Postprandial dysmetabolism plays a major role in the pathogenesis of metabolic disorders such as obesity and the polycystic ovary syndrome (PCOS). We aimed to characterize the circulating lipoprotein particle profiles in response to oral glucose, lipid and protein challenges.

Methods and results: We studied 17 women with PCOS, 17 control women and 19 healthy men selected as to have similar age and BMI. We collected blood samples following the ingestion of 300 kcal in the form of glucose, lipids, or proteins and submitted them to 2D diffusion‐ordered 1H‐NMR spectroscopy. Regardless of macronutrient administered, number of VLDL particles increased whereas LDL decreased. HDL particles increased only after lipid ingestion. Obese subjects showed an increase in the number of large VLDL particles and a decrease in large LDL particles, within a significant reduction in the average particle size of LDL. Patients with PCOS showed a particularly unfavorably smaller LDL particle size response to oral lipid intake, regardless of obesity.

Conclusions: Oral macronutrient challenges induced immediate class‐specific postprandial changes in particle number and size of lipoproteins, with lipids inducing a more pro‐atherogenic lipoprotein profile compared with glucose and proteins, particularly in obese subjects and women with PCOS.

This article is protected by copyright. All rights reserved

Publisher URL: https://onlinelibrary.wiley.com/doi/abs/10.1002/mnfr.201900928

DOI: 10.1002/mnfr.201900928

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