3 years ago

Induced Chemical Defense of a Mushroom by a Double-Bond-Shifting Polyene Synthase

Induced Chemical Defense of a Mushroom by a Double-Bond-Shifting Polyene Synthase
María García-Altares, Philip Brandt, Markus Nett, Dirk Hoffmeister, Christian Hertweck
The antilarval mushroom polyenes 18-methyl-19-oxoicosaoctaenoic acid and 20-methyl-21-oxodocosanonaenoic acid appear in response to injury of the mycelium of the stereaceous mushroom BY1. We identified a polyketide synthase (PPS1) which belongs to a hitherto completely uncharacterized clade of polyketide synthases. Expression of the PPS1 gene is massively upregulated following mycelial damage. The synthesis of the above polyenes was reconstituted in the mold Aspergillus niger as a heterologous host. This demonstrates that PPS1 1) synchronously produces branched-chain polyketides of varied lengths, and 2) catalyzes the unprecedented shift of eight or nine double bonds. This study represents the first characterization of a reducing polyketide synthase from a mushroom. We also show that injury-induced de novo synthesis of polyketides is a fungal response strategy. Shift work: The mushroom polyene synthase PPS1 produces the antilarval compounds 18-methyl-19-oxoicosaoctaenoic and 20-methyl-21-oxodocosanonaenoic acids simultaneously. PPS1 shifts the conjugated double bonds, as demonstrated by reconstituting synthase activity heterologously, and is induced by mycelial injury. The results suggest that de novo synthesis of the polyene is a chemical defense strategy of fungi.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/anie.201700767

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.