4 years ago

A Biomimetic Phosphatidylcholine-Terminated Monolayer Greatly Improves the In Vivo Performance of Electrochemical Aptamer-Based Sensors

A Biomimetic Phosphatidylcholine-Terminated Monolayer Greatly Improves the In Vivo Performance of Electrochemical Aptamer-Based Sensors
Hui Li, Jacob Somerson, Tod E. Kippin, Christina Shin, Netzahualcóyotl Arroyo-Currás, Philip A. Vieira, Philippe Dauphin-Ducharme, Shaoguang Li, Kevin W. Plaxco, Claire H. Tran
The real-time monitoring of specific analytes in situ in the living body would greatly advance our understanding of physiology and the development of personalized medicine. Because they are continuous (wash-free and reagentless) and are able to work in complex media (e.g., undiluted serum), electrochemical aptamer-based (E-AB) sensors are promising candidates to fill this role. E-AB sensors suffer, however, from often-severe baseline drift when deployed in undiluted whole blood either in vitro or in vivo. We demonstrate that cell-membrane-mimicking phosphatidylcholine (PC)-terminated monolayers improve the performance of E-AB sensors, reducing the baseline drift from around 70 % to just a few percent after several hours in flowing whole blood in vitro. With this improvement comes the ability to deploy E-AB sensors directly in situ in the veins of live animals, achieving micromolar precision over many hours without the use of physical barriers or active drift-correction algorithms. Broadcast live: A biomimetic surface employing phosphatidylcholine head groups (red spheres) greatly improves the baseline stability of electrochemical aptamer-based (E-AB) sensors in whole blood and in live rats, reducing the baseline drift from 70 % to less than 10 % under these challenging conditions. MB=methylene blue, T=target, dark blue ribbon=aptamer, yellow strip=electrode, eT=electron transfer.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/anie.201700748

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