3 years ago

Highly Hydrophilic, Two-photon Fluorescent Terpyridine Derivatives Containing Quaternary Ammonium for Specific Recognizing Ribosome RNA in Living Cells

Highly Hydrophilic, Two-photon Fluorescent Terpyridine Derivatives Containing Quaternary Ammonium for Specific Recognizing Ribosome RNA in Living Cells
Senio Campos De Souza, Qiong Zhang, Yupeng Tian, Jieying Wu, Zhongping Zhang, Hongping Zhou, Wei Du, Mingzhu Zhang, Hui Wang, Xiaohe Tian, Yingzhong Zhu, Aidong Wang
A two-photon fluorescent probe (J1) that selectively stains intracellular nucleolar RNA was screened from three water-soluble terpyridine derivatives (J1J3) with quaternary ammonium groups. The photophysical properties of J1J3 were systemically investigated both experimentally and theoretically, revealing that J1J3 possess large Stokes shifts and the two-photon absorption action cross sections range from 38 to 97 GM in the near-infrared region. This indicates that J1 could specifically stain nucleoli by targeting the nucleolar rRNA from the recognition experiments in vitro, the two-photon imaging experiments, and the stimulated emission depletion in vivo. The mechanism of action in which J1 binds to the nucleolar rRNA was researched via both experiments and molecular modeling. The high binding selectivity of J1 to nucleolar RNA over cytosolic RNA made this probe a potential candidate to visualize rRNA probe in the living cells.

Publisher URL: http://dx.doi.org/10.1021/acsami.7b08068

DOI: 10.1021/acsami.7b08068

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