4 years ago

Regulation of spinogenesis in mature Purkinȷe cells via mGluR/PKC-mediated phosphorylation of CaMKII{beta} [Neuroscience]

Regulation of spinogenesis in mature Purkinȷe cells via mGluR/PKC-mediated phosphorylation of CaMKII{beta} [Neuroscience]
Katsuhiko Mikoshiba, Naoko Ogawa, Chihiro Hisatsune, Takeyuki Sugawara, Hiroyuki Miyamoto

Dendritic spines of Purkinje cells form excitatory synapses with parallel fiber terminals, which are the primary sites for cerebellar synaptic plasticity. Nevertheless, how density and morphology of these spines are properly maintained in mature Purkinje cells is not well understood. Here we show an activity-dependent mechanism that represses excessive spine development in mature Purkinje cells. We found that CaMKIIβ promotes spine formation and elongation in Purkinje cells through its F-actin bundling activity. Importantly, activation of group I mGluR, but not AMPAR, triggers PKC-mediated phosphorylation of CaMKIIβ, which results in dissociation of the CaMKIIβ/F-actin complex. Defective function of the PKC-mediated CaMKIIβ phosphorylation promotes excess F-actin bundling and leads to abnormally numerous and elongated spines in mature IP3R1-deficient Purkinje cells. Thus, our data suggest that phosphorylation of CaMKIIβ through the mGluR/IP3R1/PKC signaling pathway represses excessive spine formation and elongation in mature Purkinje cells.

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