Journal of the American Chemical Society
Journal of the American Chemical Society
5 years ago

Rapid Microfluidic Double-Jump Mixing Device for Single-Molecule Spectroscopy

Rapid Microfluidic Double-Jump Mixing Device for Single-Molecule Spectroscopy
Stephan Benke, Fabian Dingfelder, Niels Zijlstra, Bengt Wunderlich, Franziska Zosel, Daniel Nettels, Benjamin Schuler
We introduce a microfluidic double-jump mixing device for investigating rapid biomolecular kinetics with confocal single-molecule spectroscopy. This device enables nonequilibrium dynamics to be probed, e.g., transiently populated intermediates that are inaccessible with existing single-molecule approaches. We demonstrate the potential and reliability of the method on time scales from milliseconds to minutes by investigating the coupled folding and binding reaction of two intrinsically disordered proteins and the conformational changes occurring in a large cytolytic pore-forming toxin.

Publisher URL: http://dx.doi.org/10.1021/jacs.7b02357

DOI: 10.1021/jacs.7b02357

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.