3 years ago

Evaluation of a matrix-assisted laser desorption ionization-time of flight mass spectrometry assisted, selective broth method to screen for vancomycin-resistant enterococci in patients at high risk

Susan Shin-Jung Lee, Tsi-Shu Huang, Cheng Len Sy, Bao-Chen Chen, Chiu-Yen Chen, Kuan-Sheng Wu, Chia-Chien Lee, Fang-Chen Chen

by Tsi-Shu Huang, Susan Shin-Jung Lee, Chia-Chien Lee, Chiu-Yen Chen, Fang-Chen Chen, Bao-Chen Chen, Cheng Len Sy, Kuan-Sheng Wu

Background

Bile esculin azide with vancomycin (BEAV) medium is a sensitive, but slightly less specific method for vancomycin-resistant enterococci (VRE) screening. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a rapid method for identification of clinical pathogens. This study aimed to assess the performance of a novel combination screening test for VRE, using BEAV broth combined with MALDI-TOF MS.

Materials and methods

Clinical specimens were collected from patients at risk of VRE carriage, and tested by the novel combination method, using selective BEAV broth culture method followed by MALDI-TOF MS identification (SBEAVM). The reference method used for comparison was the ChromID VRE agar method.

Results

A total of 135 specimens were collected from 78 patients, and 63 specimens tested positive for VRE positive using the ChromID VRE method (positive rate 46.7%). The sensitivity, specificity, positive predictive value, and negative predictive value of SBEAVM method after an incubation period of 28 hours were 93.7%, 90.3%, 89.4%, and 94.2%, respectively. The SBEAVM method when compared to the ChromID VRE method had a shorter turnaround time (29 vs. 48–72 hours) and lower laboratory cost ($2.11 vs. $3.23 per test).

Conclusions

This study demonstrates that SBEAVM is a rapid, inexpensive, and accurate method for use in VRE screening.

Publisher URL: http://journals.plos.org/plosone/article

DOI: 10.1371/journal.pone.0179455

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