3 years ago

Spacer capture and integration by a type I-F Cas1-Cas2-3 CRISPR adaptation complex [Biochemistry]

Spacer capture and integration by a type I-F Cas1-Cas2-3 CRISPR adaptation complex [Biochemistry]
Max E. Wilkinson, Angela Capolupo, Robert D. Fagerlund, F. Grant Pearce, Peter C. Fineran, Arjan Barendregt, Oleg Klykov, Richard A. Scheltema, Howard W. R. Maxwell, Sebastian N. Kieper, Raymond H. J. Staals, Kurt L. Krause, Albert J. R. Heck, Mihnea Bostina

CRISPR-Cas adaptive immune systems capture DNA fragments from invading bacteriophages and plasmids and integrate them as spacers into bacterial CRISPR arrays. In type I-E and II-A CRISPR-Cas systems, this adaptation process is driven by Cas1–Cas2 complexes. Type I-F systems, however, contain a unique fusion of Cas2, with the type I effector helicase and nuclease for invader destruction, Cas3. By using biochemical, structural, and biophysical methods, we present a structural model of the 400-kDa Cas14–Cas2-32 complex from Pectobacterium atrosepticum with bound protospacer substrate DNA. Two Cas1 dimers assemble on a Cas2 domain dimeric core, which is flanked by two Cas3 domains forming a groove where the protospacer binds to Cas1–Cas2. We developed a sensitive in vitro assay and demonstrated that Cas1–Cas2-3 catalyzed spacer integration into CRISPR arrays. The integrase domain of Cas1 was necessary, whereas integration was independent of the helicase or nuclease activities of Cas3. Integration required at least partially duplex protospacers with free 3′-OH groups, and leader-proximal integration was stimulated by integration host factor. In a coupled capture and integration assay, Cas1–Cas2-3 processed and integrated protospacers independent of Cas3 activity. These results provide insight into the structure of protospacer-bound type I Cas1–Cas2-3 adaptation complexes and their integration mechanism.

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