4 years ago

Three mutations switch H7N9 influenza to human-type receptor specificity

James C. Paulson, Wenli Yu, Iresha N. Ambepitiya Wickramasinghe, Marielle J. van Breemen, Ryan McBride, Robert P. de Vries, Robert J. Woods, Andrew J. Thompson, Ian A. Wilson, Cornelis A. M. de Haan, Wenjie Peng, Rogier W. Sanders, Alba T. Torrents de la Pena, Xueyong Zhu, Kim M. Bouwman, Oliver C. Grant, Monique H. Verheije

by Robert P. de Vries, Wenjie Peng, Oliver C. Grant, Andrew J. Thompson, Xueyong Zhu, Kim M. Bouwman, Alba T. Torrents de la Pena, Marielle J. van Breemen, Iresha N. Ambepitiya Wickramasinghe, Cornelis A. M. de Haan, Wenli Yu, Ryan McBride, Rogier W. Sanders, Robert J. Woods, Monique H. Verheije, Ian A. Wilson, James C. Paulson

The avian H7N9 influenza outbreak in 2013 resulted from an unprecedented incidence of influenza transmission to humans from infected poultry. The majority of human H7N9 isolates contained a hemagglutinin (HA) mutation (Q226L) that has previously been associated with a switch in receptor specificity from avian-type (NeuAcα2-3Gal) to human-type (NeuAcα2-6Gal), as documented for the avian progenitors of the 1957 (H2N2) and 1968 (H3N2) human influenza pandemic viruses. While this raised concern that the H7N9 virus was adapting to humans, the mutation was not sufficient to switch the receptor specificity of H7N9, and has not resulted in sustained transmission in humans. To determine if the H7 HA was capable of acquiring human-type receptor specificity, we conducted mutation analyses. Remarkably, three amino acid mutations conferred a switch in specificity for human-type receptors that resembled the specificity of the 2009 human H1 pandemic virus, and promoted binding to human trachea epithelial cells.

Publisher URL: http://journals.plos.org/plosone/article

DOI: 10.1371/journal.ppat.1006390

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.