5 years ago

Active Targeting of the Nucleus Using Nonpeptidic Boronate Tags

Active Targeting of the Nucleus Using Nonpeptidic Boronate Tags
Ming Wang, Rui Tang, Moumita Ray, Vincent M. Rotello, Ying Jiang, Qiaobing Xu, Ziwen Jiang
Active intracellular transport is a central mechanism in cell biology, directed by a limited set of naturally occurring signaling peptides. Here, we report the first nonpeptide moiety that recruits intracellular transport machinery for nuclear targeting. Proteins synthetically modified with a simple aromatic boronate motif are actively trafficked to the nucleus via the importin α/β pathway. Significantly, proteins too large to passively diffuse through nuclear pores were readily imported into the nucleus through this boronate-mediated pathway. The use of this simple motif to provide active intracellular targeting provides a promising strategy for directing subcellular localization for therapeutic and fundamental applications.

Publisher URL: http://dx.doi.org/10.1021/jacs.7b02801

DOI: 10.1021/jacs.7b02801

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