5 years ago

Chemoenzymatic synthesis and properties of sucuronamide

Chemoenzymatic synthesis and properties of sucuronamide
Saori Mizoguchi, Wataru Hakamata, Hiroki Hosaka, Toshiyuki Nishio, Takako Hirano, Mitsuru Tashiro

Using D-glucurono-6,3-lactone (GlcL) as a starting material, sucuronamide (SucAm) was synthesized in two steps using a chemoenzymatic method. In the first chemical synthesis, D-glucuronamide (GlcAm) was synthesized by treating GlcL with NH3 in ethanol. In the second enzymatic synthesis exploiting transfructosylation activity of β-fructofuranosidase, which is inhibited by D-glucose, from Arthrobacter sp. K-1, addition of the sucrose non-assimilating yeast, Saccharomyces bisporus NBRC1131, to the reaction mixture increased SucAm production from GlcAm and sucrose. The overall molar yield of this disaccharide after purification by column chromatography and crystallization was 34.7%.

Publisher URL: http://www.tandfonline.com/doi/full/10.1080/07328303.2017.1333122

DOI: 10.1080/07328303.2017.1333122

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.